ISSN 1729-6196

Banana and plantain embryogenic cell suspensions - Technical Guidelines 8 (1.3 Mo)
Hannelore Strosse, Régis Domergue, Bart Panis, Jean-Vincent Escalant and François Côte
Edited by Anne Vézina and Claudine Picq, 2003.
These guidelines present two protocols to produce embryogenic cell suspensions by using scalps or immature male flowers. The protocols have been developed by the Laboratory of Tropical Crop Improvement of the Katholieke Universiteit Leuven (KULeuven) and the cellular biology laboratory of the Centre de coopération internationale en recherche agronomique pour le développement (CIRAD).

ISSN 1729-6196

Global evaluation of Musa germplasm for resistance to Fusarium wilt, Mycosphaerella leaf spot diseases, and nematodes: Performance evaluation - Technical Guidelines 7 (1.1 Mo)
Jean Carlier, Dirk de Waele and Jean-Vincent Escalant
Edited by Anne Vézina and Claudine Picq, 2003.
These Technical Guidelines replace the Technical Guidelines 1 (Screening of Musa (germplasm for resistance and tolerance to nematodes) and 3 (Evaluation of Musa germplasm for resistance to Sigatoka diseases and Fusarium wilt) as far as performance evaluations are concerned.
Technical Guidelines for in-depth evaluations, which were also part of Technical Guidelines 3, are published separately. The Fusarium wilt and Mycosphaerella leaf spot sections have been updated following recommendations and comments made after analysing IMTP II results.

ISSN 1729-6196

Global evaluation of Musa germplasm for resistance to Fusarium wilt, Mycosphaerella leaf spot diseases, and nematodes: In-depth evaluation - Technical Guidelines 6 (891 Ko)
Jean Carlier, Dirk de Waele and Jean-Vincent Escalant
Edited by Anne Vézina and Claudine Picq, 2002.
These Technical Guidelines replace Technical Guidelines 1 (Screening of Musa Germplasm for resistance and tolerance to nematodes) and 3 (Evaluation of Musa germplasm for resistance to Sigatoka diseases and Fusarium wilt) as far as indepth evaluations are concerned. Technical Guidelines for performance evaluations are published separately. These changes were made to reflect the apparition of a third disease caused by a Mycosphaerella fungus (eumusae leaf spot disease), to simplify the guidelines, and to group under one cover the evaluations for the main pests and diseases affecting bananas.

ISSN 1729-6196

Cryopreservation of Musa germplasm - Technical Guidelines 5 (961 Ko)
Bart Panis and Nguyen Tien Thinh
Edited by J.V. Escalant and S. Sharrock, 2001.
This publication describes cryopreservation methods developed for Musa tissue at the Katholieke Universiteit Leuven in Belgium and the Japanese International Research Centre for Agricultural Sciences. These methodologies are specific to the type of banana tissue to be cryopreserved: individual meristems, meristem clumps (cauliflower-like structures), embryogenic cell suspensions and zygotic embryos.

ISSN 1729-6196

Post-harvest characteristics of black Sigatoka resistant banana, cooking banana and plantain hybrids - Technical Guidelines 4 (1.5 Mo)
B.K. Dadzie, 1998.
This manual is based on the results of 2.5 years (1993-1995) of research work conducted at the Fundación Hondureña de Investigación Agrícola (FHIA) in Honduras. The manual describes post-harvest characteristics at harvest, maturation, green-life, ripening quality and organoleptic features of black Sigatoka resistant hybrids.

ISSN 1729-6196

Evaluation of Musa germplasm for resistance to Sigatoka diseases and Fusarium wilt - Technical Guidelines 3 (1.18 Mo)
Gisella Orjeda, 1998.
Contains protocols for the evaluation of resistance to leaf spot and wilt diseases. Two protocols were defined to enable two levels of evaluation. The protocols were developed in response to increasing demand from numerous national programmes that wished to evaluate germplasm under local conditions and because of the need to perform in-depth studies on the pathogens and their hosts at a more limited number of locations.

ISSN 1729-6196

Routine post-harvest screening of banana/plantain hybrids: Criteria and methods Technical Guidelines 2 (1.65 Mo)
B.K. Dadzie and J.E. Orchard, 1997.
This manual describes the key post-harvest criteria and methods/procedures for routine selection of new Musa hybrids. There are many post-harvest criteria for screening new banana, cooking banana and plantain hybrids, however the major ones include : post-harvest characteristics at harvest, fruit maturation, green-life and shelf-life, fruit ripening quality, sensory quality, cooking or boiling quality, processing quality, mechanical damage, physiological disorders and post-harvest diseases.

ISSN 1729-6196

Screening of Musa germplasm for resistance and tolerance to nematodes - Technical Guidelines 1 (5.73 Mo)
P.R. Speijer and D. De Waele, 1997.
In most regions of the world, nematodes are recognized as important pests of bananas. Because banana nematodes attack the root and/or corm tissues, plant growth and yield are affected. The objective of these technical guidelines is to stimulate interest in nematode resistance and tolerance screening in bananas, and to provide a methodology for carrying out such screening: plant material, nematode inoculum, screening experiments in pots or in the field, nematode reproduction assessment, and damage assessment.

FAO/IPGRI Technical guidelines for the safe movement of germplasm: Musa spp. (2nd edition) (722 Ko)
M. Diekmann and C.A.J. Putter, 1996.
Edited by FAO/IPGRI, Rome.
These guidelines are divided into two parts. The first part makes general recommendation on how best to move Musa germplasm. The second part covers the important viral diseases of quarantine concern: abaca mosaic, banana bract mosaic, bunchy top, cucumber mosaic and banana streak.